Publications
1999
Ahn, Y., J. Sanseverino, and G.S. Sayler. 1999. "Analyses of polycyclic aromatic hydrocarbon-degrading bacteria isolated from contaminated soils. Biodegradation. 10:149-157.
Applegate, B.M., J. Shingleton, S. Ripp, N. Bright, D. Nivens, M. Simpson, and G.S. Sayler. 1999. Bioluminescent Bioreporter Integrated Circuits (BBICs) for the Detection of Toluene and Trichloroethylene (TCE) in Aqueous Solutions. In: Bioluminescence and Chemiluminescence: Perspectives for the 21st Century, eds. A. Roda, M. Pazzagli, L.J. Kricka and P.E. Stanley. p. 589-592.
Ford, C.Z., G.S. Sayler, and R.S. Burlage. 1999. Containment of a genetically engineered microorganism during a field bioremediation application. Appl. Microbiol. Biotechnol. Vol. 51, p. 397-400.
Kelly, C.J., C.A. Lajoie, A.C. Layton, and G.S. Sayler. 1999. Bioluminescent reporter bacterium for toxicity monitoring in biological wastewater treatment systems. Water Environment Research. Vol.71, No.1, p. 31-35.
Layton, A.C., B. Gregory, T.W. Schultz, and G.S. Sayler. 1999. Validation of Genetically Engineered Bioluminescent Surfactant Resistant Bacteria as Toxicity Assessment Tools. Ecotoxicol. Environ. Safety. 43:222-228.
Muccini, M, A.C. Layton, G.S. Sayler and T.W. Schultz. 1999. Aquatic toxicities of halogenated benzoic acids to Tetrahymena pyriformis. Environ. Contam. Toxicol. 62:616-622.
Nagel, A., J.T. Fleming, and G.S. Sayler. 1999. "Reduction of false positives in prokaryotic mRNA differential display. BioTechniques. Vol. 26, No. 4. p. 642-648.
Palmer, R.J., B. Applegate, R. Burlage, G.S. Sayler and D.C. White. 1999. Heterogeneity of Gene Expression and Activity in Bacterial Biofilms. In: Bioluminescence and Chemiluminescence: Perspectives for the 21st Century, eds. A. Roda, M. Pazzagli, L.J. Kricka and P.E. Stanley. p.609-612.
Sayler, G.S., C.D. Cox, R. Burlage, S. Ripp, D.E. Nivens, C. Werner, Y. Ahn, and U. Matrubutham. 1999. Field Application of a Genetically Engineered Microorganism for Polycyclic Aromatic Hydrocarbon Bioremediation Process Monitoring and Control. In Novel Approaches for Bioremediation of Organic Pollution Eds. R. Fass, Y. Flashner and S. Reuveny. p. 241-254.
1998
Applegate, B.M., S.R. Kehrmeyer, and G.S. Sayler. 1998. A Chromosomally Based tod-luxCDABE Whole-Cell Reporter for BTEX Sensing. Appl. Environ. Microbiol. Vol. 64, No. 7, p.2730-2735.
Burlage, R.S., R. Atlas, D. Stahl, G. Geesey, and G.S. Sayler, eds., Techniques in Microbial Ecology. New York: University Press, 1998. (Available only thru Oxford University Press)
Fleming, J.T., W. Yao, and G.S. Sayler. 1998. Optimization of Differential Display of Prokaryotic mRNA: Application to Pure Culture and Soil Microcosms. Appl. Environ. Microbiol. Vol. 64, No.10, p.3698-3706.
Heitzer, A., B. Applegate, S. Kehrmeyer, H. Pinkart, O. Webb, T. Phelps, D.C. White, and G.S. Sayler. 1998. Physiological considerations of environmental applications of lux reporter fusions. J. Microbiol. Methods. Vol.33 p.45-57.
Lajoie, C., A. Layton, I. Gregory, G. Sayler, D. Taylor, and A. Meyers. 1998. Molecular Analysis of Zoogloeal Clusters in Viscous Sludge. In: Water Environment Federation, 70th Annual Conference and Exposition Proceedings. Part 1 & 2, Vol. 3. p. 317-326.
Langworthy, D.E., R. D. Stapleton, G.S. Sayler and R.H. Findlay. 1998. Genotypic and Phenotypic Responses of a Riverine Microbial Community to Polycyclic Aromatic Hydrocarbon Contamination. Appl. Environ. Microbiol. Vol. 64, No. 9, p. 3422-3428.
Layton, A.C., M. Muccini, M. Ghosh, and G.S. Sayler. 1998. The Construction of a Bioluminescent Reporter Strain to Detect Polychlorinated Biphenyls. Appl. Environ. Microbiol. Vol. 64, No. 12, p.5023-5026.
Matrubutham, U. and G.S. Sayler. 1998. Microbial biosensors based on optical detection. In: Methods in Biotechnology, Vol. 6: Enzyme Biosensors: Techniques and Protocols. Eds. A. Mulchandani and K.R. Rogers. Humana Press Inc., New Jersey. p.249-256.
Nadeau, L.J., G.S. Sayler, and J.C. Spain. 1998. Oxidation of 1,1,1-trichloro-2,2-bix(4-chlorophenyl) ethane (DDT) by Alcaligenes eutrophus A5. Arch. Microbiol. 171:44-49.
Robinson, K.G., J.G. Pieters, J. Sanseverino, C.D. Cox, C. L. Wright, C.L. Cheng, and G.S. Sayler. 1998. Microbial oxidation and bioluminescence response for toluene and trichloroethylene. Wat.Sci.Tech. 38:7, p.1-8.
Sayler,G.S. 1998. Innovations and Broad Horizons for Environmental
Biotechnology. Australian Biotechnology, Vol. 8, No. 4., p.228-234.
Layton, A.C., C.A. Lajoie, J.P. Easter, M. Muccini, and G.S. Sayler.
1998. An integrated surfactant solubilization and PCB bioremediation
process for soils. Bioremediation Journal. 2(1):43-56.
Schultz, T.W., D.H. Kraut, G.S. Sayler, and A. Layton. 1998. Estrogenicity of Selected Biphenyls Evaluated using a Recombinant Yeast Assay. Environ. Toxicol. Chem. Vol. 17, No. 9, p. 1727-1729.
Shi, Z., K.A. LaTorre, M.M. Ghosh, A.C. Layton, S.H. Luna, L. Bowles and G.S. Sayler. 1998. Biodegradation of UV-Irradiated Polychlorinated Biphenyls in Surfactant Micelles. Wat. Sci. Tech. Vol. 38, No. 7, pp. 25-32.
Shingleton, J.T., B.M. Applegate, A.C. Nagel, P.R. Bienkowski, G.S. Sayler. 1998. Induction of the tod Operon by Trichloroethylene in Pseudomonas putida TVA8. Appl. Environ. Microbiol. Vol. 64, No. 12, p. 5049-5052.
Simpson, M.L., G.S. Sayler, B.M. Applegate, S. Ripp, D.E. Nivens, M.J. Paulus, and G.E. Jellison Jr. 1998. Bioluminescent-Bioreporter Integrated Circuits form Novel Whole-Cell Biosensors. Trends in Biotechnology. Vol.16, p.332-338.
Stapleton, R.D., S. Ripp, L. Jimenez, S. Koh, J.T. Fleming, I.R. Gregory, and G.S. Sayler. 1998. Nucleic Acid Analytical Approaches In Bioremediation: Site Assessment and Characterization. J. Microbiol. Methods. Vol.32, p.165-178.
Stapleton, R.D., D.C. Savage, G.S. Sayler, and G. Stacey. 1998. Biodegradation of Aromatic Hydrocarbons in an Extremely Acidic Environment. Appl. Environ. Microbiol. Vol. 64, No. 11, p. 4180-4184.
Stapleton, R. and G. Sayler. 1998. Assessment of the Microbiological Potential for the Natural Attenuation of Petroleum Hydrocarbons in a Shallow Aquifer System. Microbial Ecology. 36:349-361.
1997
Applegate, B., C. Kelly, L. Lackey, J. McPherson, S. Kehrmeyer, F. Menn, P. Bienkowski, and G. Sayler. 1997. Pseudomonas putida B2: A tod-lux bioluminescent reporter for toluene and trichloroethylene cometabolism. J. Industrial. Microbiol. 18:4-9.
Beck, M.J., A.C. Layton, C.A. Lajoie, J.P. Easter, G.S. Sayler, J. Barton, and M. Reeves. 1997. An integrated treatment system for polychlorinated biphenyls remediation. . In: Biotechnology in the Sustainable Environment. Eds. G. Sayler, J. Sanseverino, and K. Davis. Plenum Press. p. 73-84.
Ganesh, R., K.G. Robinson, G.D. Reed, and G.S. Sayler. 1997 Reduction of Hexavalent Uranium from Organic Complexes by Sulfate and Iron Reducing Bacteria. Appl. Environ. Microbiol. Vol. 63, No. 11, p. 4385-4391.
Lajoie, C.A., A.C. Layton, J.P. Easter, F. Menn, and G.S. Sayler. 1997. Degradation of nonionic surfactants and polychlorinated biphenyls by recombinant field application vectors. J. Industrial Microbiol. Biotechnol. Vol. 19, p. 252-262.
Lajoie, C.A., A.C. Layton, R.D. Stapleton, I.R. Gregory, A.J. Meyers, and G.S. Sayler. 1997. Molecular analysis and control of activated sludge. In: Biotechnology in the Sustainable Environment. Eds. G. Sayler, J. Sanseverino, and K. Davis. Plenum Press. p. 323-342.
Matrubutham, U., J.E. Thonnard, and G.S. Sayler. 1997 Bioluminescence Induction Response and Survival of the Bioreporter Bacterium Pseudomonas fluorescens HK44 in Nutrient Deprived Condition. Appl. Microbiol. Biotech. 47:604-609.
Palmer, Jr., R.J., C. Phiefer, R. Burlage, G. Sayler, D.D. White. 1997. Single-cell bioluminescence and GFP in biofilm research. Biolumin. Chemilumin., Proc. Int. Symp., 9th p. 445-450.
Pieters, J.G., J. Sanseverino, C.L. Wright, C.L. Cheng, K. Liu, K.G. Robinson, C.D. Cox, S.R. Kehrmeyer, B.M. Applegate, and G.S. Sayler. 1997. Bioluminescence and Cometabolic Degradation of Toluene and Trichloroethylene By Pseudomonas Putida TVA8. Proceedings of 52nd Purdue Ind. Waste Conf.. West Lafayette, IN.
Sayler, G.S. 1997. Challenges and Opportunities in the Area of Environmental Biotechnology. In: Biotechnology in the Sustainable Environment. Eds. G. Sayler, J. Sanseverino, and K. Davis. Plenum Press. p. 1-4.
Sayler, G.S. 1997. Molecular Environmental Diagnostic Applications in Hazardous Waste Bioremediation. Proceedings of the First International Symposium, Institute of Environmental Technology and Industry (IETI). Pusan National University, Korea.
Sayler, G.S., U. Matrubutham, F. Menn, W. Johnston, and R. Stapleton. 1997. Molecular Probes and Biosensors in Bioremediation and Site Assessment. In: Bioremediation-Principles and Practice. Ed. S.K. Sikdar and R.L. Irvine. Technomic Publishing Co., Inc. p.385-420
Sayler, G.S., C. Steward, U. Matrubutham, G. Huxel, J. Thonnard, and J. Drake. 1997. A Species Invasion Paradigm for Managing Biodegradative Microbial Communities. In: Progress in Microbial Ecology: Proceedings for the 7th International Symposium on Microbial Ecology (ISME-7), Brazil. p. 577-585 .
Webb, O.F., P.R. Bienkowski, A. Heitzer, and G.S. Sayler. 1997. Kinetics and Response of a Pseudomonas fluorescens HK44 Biosensor. Biotech. Bioengineering. Vol. 54, No. 5, pp491-502.
1996
Bowman, J. and G. Sayler. 1996. Nucleic acid techniques in the environmental detection of microorganisms and their activities. In: Mol. Approaches Environ. Microbiol. Eds. Pickup, R.W., Sounders, J.R., p. 63-67. Horwood, London, UK.
Burlage, R.S., Z. Yang, R.J. Palmer, G.S. Sayler, and Y. Khang. 1996. Development and Application of the Lux Gene for Environmental Bioremediation. Proceedings SPIE Meeting on Advanced Technologies for Environmental Monitoring and Remediation, 8/96, Denver, Co. (PDF format available)
Johnston, W.H., R. Stapleton, and G.S. Sayler. 1996. Direct extraction of microbial DNA from soils and sediments. In: Molecular Microbial Ecology Manual. Ed. A.D.L. Akkermans, J.D. van Elsas and F.J. de Bruijn. Kluwer, Netherlands. 1.3.2:1-9.
Tschantz, M.F., J.P. Bowman, F. Evans, P.R. Bienkowski, T.L. Donaldson, and G.S. Sayler. 1996. Development of a fully suspended, multi-stage bioreactor system for trichloroethylene degradation based on soluble monooxygenase cometabolism. Environ. Sci. Health, A31(1), p. 249-266.
White, D.C., A.A. Arrage, D.E. Nivens, R.J. Palmer, J.F. Rice, and G.S. Sayler. 1996. Biofilm ecology: On line methods bring new insights into mic and microbial biofouling. Biofouling, 10(1-3), 3-16.
1995
Applegate, B., U. Matrubutham, J. Sanseverino, and G.S. Sayler. 1995. Biodegradation genes as marker genes in microbial ecosystems. In: Molecular Microbial Ecology Manual. Ed. A.D.L. Akkermans, J.D. van Elsas and F.J. de Bruijn. Kluwer, Netherlands. 6.1.8:1-14.
Bowman, J., J. Fleming, I.R. Gregory, A. Ogram, G. Sayler, W. Sun, and D. Zhang. 1995. Nucleic acid technology report for the in situ bioremediation demonstration (methane biostimulation) of the Savannah River Integrated Demonstration Project DOE/OTD, Pacific Northwest Laboratory.
Fleming, J.T. and G.S. Sayler. 1995. Assessment of Gene Expression in the Environment: Quantitative mRNA Analysis in Contaminated Soils. In: Applications of Molecular Biology in Environmental Chemistry. Ed. R.A Minear, A. Ford, L. Needham, and N. Karch. CRC Press, p. 45-58.
Heitzer, A., O.F. Webb, P.M. DiGrazia and G.S. Sayler. 1995. A Versatile Bioluminescent Reporter System for Organic Pollutant Bioavailability and Biodegradation. In: Applications of Molecular Biology in Environmental Chemistry. Ed. R.A Minear, A. Ford, L. Needham, and N. Karch. CRC Press, p. 191-208. (PDF format available)
Kehrmeyer, S.R., B.M. Applegate, H.C. Pinkart, D.B. Hedrick, D.C. White, and G.S. Sayler. 1995. Combined Lipid/DNA Extraction Method for Environmental Samples. J. Microbiol. Methods. 25:153-163.
Rice, J.F., R.F. Fowler, J.T. Fleming, A.A. Arrage, D.C. White and G.S. Sayler. 1995. Effects of External Stimuli on Environmental Bacterial Strains Harboring an algD-lux Bioluminescent Reporter Plasmid for the Study of Corrosive Biofilms. J. Indust. Microbiol. 15:318-328.
Sayler, G. 1995. Biodegradation Process Analysis: Molecular Application in Simulations and Environmental Verification. In: Proceedings Environmental Biotechnology. Ed. I. Economidis and A. Palmisano. EC-US Task Force on Biotechnology Research. Brussels, Belgium p.47-54.
Sayler, G. 1995. Bioluminescence Reporter Genes for Monitoring Biodegradative Activities. Proceedings: Bioremediation: The Tokyo ’94 Workshop. Organization for Economic Cooperation and Development Tokyo, Japan.
Sayler G.S., A. Layton, C. Lajoie, J. Bowman, M. Tschantz, and J.T. Fleming. 1995. Molecular Site Assessment and Process Monitoring in Bioremediation and Natural Attenuation. Appl. Biochem. Biotech. 54:277-290.
Sayler, G.S., U. Matrubutham, R. Palmer, and C. Kelly. 1995. Application of Molecular Biology to Real Time Monitoring in Bioremediation. Proceedings for the OECD Workshop Amsterdam 1995 on Wider Applications and Diffusion of Bioremediation Technologies. p. 323-334.
Sayler, G.S., U. Matrubutham, C. Steward, A. Layton, C. Lajoie, J. Easter, and B. Applegate. 1995. Towards Field Release of Engineered Strains for Bioremediation. Proceedings of the 7th Symposium on Environmental Releases of Biotechnology Products: Risk Assessment Methods and Research Progress. ORD/U.S. Environmental Protection Agency, Gulf Breeze, FL.
Sayler, G. and P. Sayre. 1995. Preliminary Risk Assessment for Recombinant Pseudomonads Released into the Environment for Hazardous Waste Degradation. Proceedings:. Bioremediation: The Tokyo ’94 Workshop. Organization for Economic Cooperation and Development Tokyo, Japan.
Stapleton, R.D. and G.S. Sayler. 1995. Biodegradative Potential for Natural Attenuation of Hydrocarbons in a Shallow, Unconfined Aquifer Field Test Site. Proceedings of the 88th annual Meeting of the Air and Waste Management Association, San Antonio, TX.
Tschantz, M.F., J.P. Bowman, T.L. Donaldson, P.R. Bienkowski, J.M. Strong-Gunderson, A.V. Palumbo, S.E. Herbes and G.S. Sayler. 1995. Methanotrophic TCE Biodegradation in a Multi-Stage Bioreactor. Environ. Sci. Tech. 29.8:2073-2082.